We offer design of universal primers for DNA regions (for example, gene exons) by your individual order. The primers design enables sequencing with the use of M13 universal primers included into the kit. It gives the opportunity to dramatically reduce tests labor intensity and perform sequencing under the same conditions for the most of amplicons.
The kits are analogous to ABI BigDye Direct Cycle Sequencing Kit. Utilizing the kits eliminates the necessity to optimize the reaction conditions or select specific primers for sequencing. The only need is to clean PCR product (usually even gel purification is not required), start sequencing reaction with the BigDye Terminator Cycle Sequencing Kit (v1.1 or v3.1) using enclosed M13 primers (which are always the same for all amplicons) and conduct capillary electrophoresis sequencing.